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WORKSHOP 1: qPCR DETECTION OF HUMAN PAPILLOMAVIRUS 16-DNA IN PATIENTS WITH LARYNGEAL CARCINOMA

Number of participants: 10

Moderator: Tihomir Rashev

Department of Molecular Biology, Medical University - Pleven, Bulgaria

METHODS AND PROCEDURES

  • Sample collection: clinical materials (tissue samples with carcinoma of the larynx, all histologically confirmed) – dr Svetla Pencheva (Department of anatomy histology and cytology).
  • Viral DNA extraction with DNA-sorb-C nucleic acid extraction kit AmpliSens®-US
  • DNA detection via Primerdesign Human Papillomavirus 16–E6 gene genesig®Advanced Kit –this step will be done by the participants in the workshop.

PCR

We prepared the mix to run the qPCR Genotyping Master mix and Genotyping Assay and added the samples

  • For each DNA sample prepare a reaction mix according to the table below: Include sufficient reactions for positive and negative controls.
  • Pipette 15μl of each mix into individual wells according to your real-time PCR

experimental plate set up.

  • Prepare sample DNA templates for each of your samples.
  • Pipette 5μl of DNA template into each well, according to your experimental plate set up. For negative control wells use 5μl of RNAse/DNAse free water. The final volume in each well is 20μl.

Reaction components

96-Well Fast Plate ( 10µL reaction)

PrecisionPLUSTM 2x qPCR MasterMix

10 µL

HPV16 primer mix

1 µL

Extraction control primer

1 µL

RNAse/DNAse free water

3 µL

DNA templates

5 µL

Total volume per Well

20 µL

 

AMPLIFICATION PROTOCOL

We performed the PCR:

Step

Custopm

Temperature

Duration

Cycles

AmpliTaq Gold, UP, Enzyme Activation

95 ˚C

10 minutes

HOLD

Denaturation

95 ˚C

15 seconds

50

Annealing/Extension

60 ˚C

1 minute

Data collection

60 ˚C

1 minute

  • Data analysis: the Pico Real Software analyzed the results, the presence or absence of fluorescence and the allelic discrimination automatically
  • The initial preliminary studies will be sent to the participants via e-mail

WORKSHOP 2: ANESTHESIOLOGY: AIRWAY  MANAGEMENT

Number of participants: 40

Moderator: Petko Stefanovski, MD

UMHAT “Dr Georgi Stranski”, Pleven, Bulgaria

Every doctor, no matter what specialty they practice, must be familiar with the indications and techniques for intervention in the respiratory system. The main approach involves providing clear airways, protection from aspiration and ensuring adequate oxygenation and ventilation. This workshop describes the steps involved in assessing the airways, ensuring the rapid implementation of endotracheal intubation (RSI), as well as the necessary steps when it comes to cases with unsuccessful or hard intubation.


WORKSHOP 3: SURGICAL  SUTURES  AND  KNOTS

Number of participants: 30

Special requirements: Participants should have finished 3rd year of studies!

Moderator: Emil Filipov, MD

UMHAT “Dr Georgi Stranski”, Pleven, Bulgaria

Surgical suturing and knot tying are one of the basic medical procedures which any doctor should be able to perform. This workshop will give the participants opportunity to practice different types of surgical sutures and different techniques for tying surgical knots.


WORKSHOP 4: INHALATION  DEVICES  IN  PULMONOLOGY.  CORRECT  INHALATION  TECHNIQUE

Number of participants: 30

Moderator: Zlatina Ivanova, MD*, Nikolay Kyuchukov, MD, PhD*

Department of Cardiology, Pulmonology, Endocrinology, UMHAT “Dr Georgi Stranski”, Pleven, Bulgaria

Inhalation remains the most effective way to treat obstructive pulmonary diseases. Good inhalation technique leads to good control on the obstructive disease, rarer use of reliever medications, higher lung volumes and better quality of life. Most of the patients cannot use their inhaler device properly.

The aim of the workshop is to present the most common types of inhalation devises, their correct usage and the methods for assessing the inhalation technique.

The participants will have the opportunity to test their inhalation technique via Vitalograph AIM devise.  The workshop is suitable medical students, nurses and rehabilitators who passed their Pulmonology course. After the workshop the participants will be able to assess the inhalation technique, to correct the mistakes and to provide adequate advises to the patients. The optimal number of the participants in the workshop is 18.


WORKSHOP 5: ORTHOPEDIC  CAST – TYPES,  TECHNIQUE  AND  APPLICATION

Number of participants: 20

Special requirements: Participants should have finished 3rd year of studies!

Moderator: Assoc. Prof. Svilen Todorov, MD, PhD

Department of Orthopedics and Traumatology, UMHAT “Dr Georgi Stranski”, Pleven, Bulgaria

PART I: THEORETICAL PART (20 min)

It will explain the main indications for orthopedic cast in the traumatology practice. It will also explain techniques for application.

PART II: PRACTICAL PART

The participants will have the chance to try the main techniques for application and removal of the cast from limbs.


WORKSHOP 6: INVASIVE  DIAGNOSTIC  PROCEDURES  DURING  PREGNANCY.  HOW  TO  LEARN  TO  DO  CHORION  BIOPSY,  AMNIOCENTESIS  AND  FETAL  SURGERY  UNDER  ULTRASOUND  CONTROL

Number of participants: 15

Special requirements: Participants should have finished 4th year of studies!

Moderator: Petya Chaveeva, MD

Fetal Medicine Specialist, Medical Complex “Shterev, MD”, Sofia, Bulgaria

The aim of the workshop is to provide students with the opportunity to learn about medical procedures during pregnancy. In the timeframe of the workshop they will have full access to the available information on how to do such procedures and what are the rules and techniques needed to perform them during pregnancy. Participants will have the opportunity to get acquainted with the tools and principles, and try to apply the procedure on a “model” that imitates pregnancy.


WORKSHOP 7: EVALUATION  OF  CARDIOVASCULAR  RISK

PART I: THEORETICAL PART

THE  GLOBAL  CARDIOVASCULAR  RISK  ESTIMATION

Assoc. Prof. Plamen Gatzov, MD, PhD

Department of Invasive Cardiology, Lozenetz Hospital, Sofia, Bulgaria

Cardiovascular diseases (CVD) are the major cause for morbidity and mortality in the European and North American countries. The arterial hypertension, atherosclerosis and its consequences – the ischemic heart disease (IHD), cerebrovasvular disease (CVD), and the arterial insufficiency in other organs and systems – extremities, organs in the abdomen et cetera, are the reasons for frequent hospitalizations, high mortality, and high human and financial expenses in the modern society.  The risk factors for atherosclerosis are many, and the age, male gender, arterial hypertension, diabetes mellitus, smoking and hypercholesterolemia are only some of them. The presence of more risk factors in one individual increase the risk for atherosclerosis and its consequences. The proper influence on the manageable risk factors leads to disease prevention. In the medical journals, practical guidelines for cardiovascular risk estimation, based on the presence and severity of risk factors, are published periodically. Nomograms for cardiovascular disease risk estimation in every particular patient have been developed. They have been published in the guidelines and imply a reference for the practicing physician to inform the patient about the risk degree and to develop a program for risk reduction and disease prevention. The prevention and cardiovascular disease reduction in the population is a multifactorial task, which includes the efforts of the health system, governmental institutions, physicians, patients and the entire society.

DYSLIPIDEMIA  AND  CARDIOVASCULAR  RISK.  ARGUMENTS  FOR  INITIATING  DECREASING  THE  LEVEL  OF  LIPOPROTEINS

Prof. Maria Tzekova, MD, PhD, DSc
Department of Cardiology, Pulmonology, Endocrinology, UMHAT “Dr. Georgi Stranski”, Pleven, Bulgaria

Dyslipidemia is a disorder characterized by abnormalities of concentration, size or components of uncontrolled levels of lipoprotein in the blood. These abnormalities are primary (genetic) causes. Secondary risk factors are clinical and environmental factors as well, worsening this condition; either increased synthesis or decreased breaking of lipoproteins. These risk factors indicate progression of atherogenesis. Atherogenesis tends to be a slowly progressive disorder that hardens the early diagnostic process. The following stages in progression are the onset of severe complications as coronary artery disease, as cerebrovascular disease as peripheral arterial disease). The Framingham Risk Score is one of a number of scoring systems used to determine an individual's chances of developing cardiovascular disease. Dyslipidemia has been proved in 47% of patients with coronary artery disease and 35% of ID patients (Woodward M.,2008). Treatment of dietary changes and lipid lowering drugs, called statins, can potentially delay the progression of LDL-C increasing. This decreases the risk of heart attacks. Recent literature has provided changes for the aim of treatment and also CHANGES in target levels of LDL-C due to the stage of cardiovascular risk. Physicians recommend a more intense treatment for patients who are more at risk of cardiovascular diseases; further more physicians recommend reaching those LDL-C target levels as close as possible.

PART II: PRACTICAL PART

Moderator: Orlin Asenov, MD