Prof. Maksim Pogorielov, MD, PhD, DMSc

Deputy Director for Science, Medical Institute, Sumy State University, Sumy, Ukraine

 

Organ transplantation has been saving the lives of millions of patients that have incurable diseases since the 1950s, but the demand for this procedure has far surpassed the number of donors available. An alternative needs to be provided to satisfy the increasing number of patients waiting to receive transplantation. Additive manufacturing techniques offer the potential to fabricate organized tissue constructs to repair or replace damaged or diseased human tissues and organs. 3D bioprinting overcomes the disadvantages of scaffold-based tissue engineering technologies, such as restriction of structural complexity and spatial heterogeneities by printing bioink layer-by-layer, in a scaffold-free fashion to mimic the natural structure of the target tissue.

 

A 3D bioprinted tissue or organ is either incubated in a bioreactor in vitro for maturation before implantation via surgery, or printed in situ in which case the human body acts as the bioreactor. The most common 3D bioprinting systems are laser direct writing, inkjet extrusion, and cell electrospinning.

 

In current lecture we provide detailed description, advantages and disadvantages of different 3D bioprinting technologies. Information about bioink compounds, their properties and application will be described. Cell source, advantages of primary, stem cells and iPSCs cells will be mentioned in current lecture.

 

Selected application of 3D technologies and bio-electrospinning will be considered, including cardiac, bone, neural and skin tissues regeneration.